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1 year ago
1 year ago by Jesualdo Hernández
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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|The other part of the cornea was used for protein extraction. The corneas were weighed and separately chopped into small pieces. The tissue was solidified by immersion in liquid nitrogen and then ground into a powder. This step was repeated three times. Tissue extraction was performed according to the reagent instructions (Tissue Protein Extraction Cocktail, ThermoFisher Scientific, Inc., Waltham, MA, USA).|