DNA gel extraction / PCR product purification

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human osteoblasts

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized PC-12

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized PC-3

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary mouse oocytes