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Get tips on using pwPICZalpha-DT390-bi-pIL-2-Non-N-Gly to perform Protein Expression Eukaryotic cells - P. pastoris Porcine IL-2 fusion toxins

Products Zhirui Wang, Transplantation Biology Research Center, Massachuse pwPICZalpha-DT390-bi-pIL-2-Non-N-Gly

Get tips on using Flp-In™ T-REx™ 293 Cell Line to perform Protein expression and purification Mammalian cells - CAL-51 BRCA1

Products Thermo Fisher Scientific Flp-In™ T-REx™ 293 Cell Line

Get tips on using Jump In™ T-REx™ HEK 293 Kit to perform Protein expression and purification Mammalian cells - HEK 293 HER2

Products Thermo Fisher Scientific Jump In™ T-REx™ HEK 293 Kit

Get tips on using pMT/BiP/V5-His A, B, & C Drosophila Expression Vectors to perform Protein expression and purification Insect cells - S2 HER2

Products Thermo Fisher Scientific pMT/BiP/V5-His A, B, & C Drosophila Expression Vectors

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - PC-12

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - PC-9

Products Sigma-Aldrich Cell Proliferation Kit I (MTT)

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type PC12

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - PC-9

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - PC-3

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - PC-12

Products Sigma-Aldrich Anti-p62/SQSTM1 antibody produced in rabbit

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