Cell cytotoxicity / Proliferation assay

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Hep G2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Capan-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay SJSA-1

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HEK 293T

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - human Mesenchymal stem cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - T47D

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - HepG2

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - A549