Get tips on using 300 prep FavorPrep™ Plasmid DNA Extraction Mini Kit (sample size: 1~ 5 ml culture cells) to perform Plasmid Isolation Vibrio parahaemolyticus
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized Sf9
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized PNT2
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HepG2
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HaCaT
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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