siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Escherichia coli

Get tips on using Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA to perform ELISA Rat - SOD2/Mn-SOD

Get tips on using STEMdiff™ SMADi Neural Induction Kit to perform Stem cell Differentiation media Differentiation of Human iPSC into Human Neuroepithelial cells

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human esophageal organoids

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human liver organoids