siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody to perform Immunohistochemistry Mouse - Gfrα2

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - GH3

Get tips on using EZ4U - Cell Proliferation Assay to perform Live / Dead assay mammalian cells - GH3

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HeLa

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media Human bone mesenchymal stem cell (BMSC)

Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)