RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Hippocampus

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Esophagus

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Epididymis

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Decidua

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Colon

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Bone

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Adipose

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.