Immunohistochemistry Collagen Type III Goat Mouse

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Get tips on using CellGenix® GMP SCGM Stem Cell Growth Medium to perform Mammalian cell culture media NK-92

Products CellGenix CellGenix® GMP SCGM Stem Cell Growth Medium

Get tips on using GATA-1 shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 GATA‐1

Products Santa Cruz Biotechnology GATA-1 shRNA Plasmids (h)

Get tips on using GATA-4 Antibody (G-4): sc-25310 to perform Western blotting GATA-4

Products Santa Cruz Biotechnology GATA-4 Antibody (G-4): sc-25310

Get tips on using GATA-3 Antibody (HG3-31): sc-268 to perform ChIP Anti-bodies GATA3

Products Santa Cruz Biotechnology GATA-3 Antibody (HG3-31): sc-268

Get tips on using Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) to perform ChIP Anti-bodies GATA3

Products Abcam Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

DNA CRISPR Rat Deletion PC12 myosin IIA (Myh9)

Get tips on using siGENOME Rat Gata1 (25172) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) Gata1

Products Horizon Discovery Ltd. siGENOME Rat Gata1 (25172) siRNA - SMARTpool

Get tips on using TRI Reagent® to perform Protein isolation Mammalian cells - Mouse_Brown fat

Products Sigma-Aldrich TRI Reagent®

Get tips on using Gateway™ pDONR™221 Vector to perform Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5

Products Thermo Fisher Scientific Gateway™ pDONR™221 Vector

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Mammalian cells Mouse_Brown fat

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