siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using EpiTect Fast 96 Bisulfite Kit to perform Bisulfite DNA Modification Cell lines / primary cells

Get tips on using EpiTect Plus DNA Bisulfite Kit to perform Bisulfite DNA Modification Cell lines / primary cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Human Dental pulp stem cells (hDPSC)

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media Human Tendon Stem/Pluripotence cells (TSPCs)

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine monocyte derived macrophages

Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Cells - primary mouse ventricles

Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Cells - primary mouse cardiomyocytes

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary rat astrocytes

Get tips on using HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2 to perform shRNA gene silencing Mouse - FL83B HtrA2