siRNA / miRNA gene silencing Human PC-3

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Human - OSCC

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - PBMC

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - LCL

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - HUVEC

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - HepG2

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Human - HeLa

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - T47D

Get tips on using HSP70 High Sensitivity ELISA kit to perform ELISA Human - HSP70

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.