ChIP acH3 Canine Human

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using PARP-1 C-terminal antibody (pAb) to perform ChIP Anti-bodies PARP

Get tips on using Recombinant Anti-PARP1 antibody [E102] (ab32138) to perform ChIP Anti-bodies PARP

Get tips on using Anti-RPA32/RPA2 antibody [9H8] (ab2175) to perform ChIP Anti-bodies RPA

Get tips on using Sall4 Antibody (EE-30): sc-101147 to perform ChIP Anti-bodies Sall4

Get tips on using Anti-Estrogen Receptor Antibody, clone AER304 to perform ChIP Anti-bodies ERα

Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP Anti-bodies H3K9me3

Get tips on using Anti-trimethyl-Histone H3 (Lys27) Antibody to perform ChIP Anti-bodies H3K27me3

Get tips on using Anti-dimethyl-Histone H3 (Lys27) Antibody to perform ChIP Anti-bodies H3K27me2

Get tips on using Anti-monomethyl-Histone H3 (Lys27) Antibody to perform ChIP Anti-bodies H3K27me1