When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36
Get tips on using Stealth siRNA(r)_Plat to perform siRNA / miRNA gene silencing Rat - NPC tPA/Plat
Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa
Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes
Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines COS7
Get tips on using Targefect-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment