Site Directed Mutagenesis (SDM) Human Deletion MCF-7

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - U87

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - Jurkat

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - K562

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SKOV3

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Kupffer Cells

Get tips on using SOLiD™ ChIP-Seq Kit, with ChIP magnet to perform ChIP Human - SH-SY5Y

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Human - BMDM

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2