A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using Penta·His Biotin Conjugate to perform Protein tag Detection of His-tagged proteins
Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA
Get tips on using Penta·His HRP Conjugate Kit to perform Protein tag Detection of His-tagged proteins
Get tips on using RGS·His HRP Conjugate Kit to perform Protein tag Detection of His-tagged proteins
Get tips on using Penta·His Alexa Fluor 647 Conjugate to perform Protein tag Detection of His-tagged proteins
Get tips on using Penta·His Alexa Fluor 488 Conjugate to perform Protein tag Detection of His-tagged proteins
Get tips on using Cox-2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 COX-2
Get tips on using connexin 43 ShRNA to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment