Get tips on using LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy to perform Live / Dead assay bacteria - Porphyromonas gingivalis
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB
Get tips on using pMCSG7-D1aerobic to perform Protein Expression Prokaryotic cells - E. coli D1
Get tips on using Prestained Dual Color Protein Molecular Weight Marker (1.7-40 kDa) Molecular Weight Marker to perform Protein Ladder Prestained
Get tips on using pCX-puro-mouLH to perform Protein Expression Eukaryotic cells - CHO mouse LH
Get tips on using pCX-puro-mouFSH to perform Protein Expression Eukaryotic cells - CHO mouse FSH
Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation interferon-γ promoter
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment