Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP)

Get tips on using The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Get tips on using QIAGEN Protease (30 AU) to perform Digestion during DNA preparation

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using RNeasy Protect Cell Mini Kit to perform RNA isolation / purification Cells - immortalized C6/36

Get tips on using RNeasy Protect Saliva Mini Kit (50) to perform RNA isolation / purification Tissue - Human Saliva

Get tips on using RNeasy Protect Bacteria Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using RNeasy Protect Bacteria Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis

Get tips on using RNeasy Protect Cell Mini Kit (50) to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay mammalian cells - FE002-SK2 human skin progenitor cells

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.