shRNA gene silencing Human SiHa

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DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC) Experiment

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat aortic smooth muscle cells (rASMC)

RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus Experiment

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus aureus

RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pneumoniae

RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis Experiment

RNA RNA isolation / purification Bacteria Gram positive Mycobacterium tuberculosis

RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis Experiment

RNA RNA isolation / purification Bacteria Gram positive Enterococcus faecalis

RNA isolation / purification Bacteria - Gram positive Bacillus anthracis Experiment

RNA RNA isolation / purification Bacteria Gram positive Bacillus anthracis

RNA isolation / purification Bacteria - Gram positive Clostridium difficile Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium difficile

RNA isolation / purification Bacteria - Gram positive Clostridium tetani Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium tetani

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridum botulinum

RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus Experiment

RNA RNA isolation / purification Bacteria Gram positive Lactobacillus amylovorus

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