Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rabbit bone marrow mesenchymal stem cells
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse liver tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse liver tissue
Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T
Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - LTEP-a-2 lung adenocarcenoma
Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human liver organoids
Get tips on using Cell Counting Kit-8 (CCK-8) to perform Live / Dead assay mammalian cells - MMQ
Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma
Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells
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