siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

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Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rabbit bone marrow mesenchymal stem cells

Products Abcam Live and Dead Cell Assay (Abcam)

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type T-cells Mouse (CD4+ and CD8+)

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse liver tissue

Products Sigma-Aldrich In Situ Cell Death Detection Kit, Fluorescein

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse liver tissue

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T

Products Sigma-Aldrich Cell Proliferation Reagent WST-1

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - LTEP-a-2 lung adenocarcenoma

Products Sigma-Aldrich Cell Proliferation Kit I (MTT)

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human liver organoids

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

Get tips on using Cell Counting Kit-8 (CCK-8) to perform Live / Dead assay mammalian cells - MMQ

Products BosterBio Cell Counting Kit-8 (CCK-8)

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Products Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay

Get tips on using PolyFect Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells

Products Qiagen PolyFect Transfection Reagent

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