ChIP acH3 Rat Sheep BEF Tag

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Mouse - RAW264.7

Get tips on using ATG12 Antibody (166) to perform Autophagy assay cell type - RAW 264.7

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd

Get tips on using Cytokeratin 19 Antibody to perform Immunohistochemistry Cytokeratin 19 - Rabbit Mouse -NA-

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media RAOSMC

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

Get tips on using Anti-Ki-67 Antibody to perform Immunohistochemistry Ki67 - Rabbit Mouse -NA-

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.