Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - PANC-1
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MCF-7
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - SH-SY5Y
Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 PKN3
Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b
Get tips on using LC3B Antibody to perform Autophagy assay cell type - Rat brain homogenate
Get tips on using PI/RNASE Solution to perform Cell cycle assay human - U266
Get tips on using PI/RNASE Solution to perform Cell cycle assay human - Jurkat
Get tips on using PI/RNASE Solution to perform Cell cycle assay human - K562
Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
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