pcr-multiplex-pcr-mammalian-dna

Get tips on using DraI (10 U/µL) to perform Restriction Enzymes DraI

Get tips on using DraI (AhaIII) restriction enzyme to perform Restriction Enzymes DraI

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using Anti-PCNA antibody [PC10] (ab29) to perform Immunohistochemistry Mouse - PCNA

Get tips on using PCNA Antibody (PC10): sc-56 to perform Immunohistochemistry Mouse - PCNA

Get tips on using PCNA (PC10) Mouse mAb #2586 to perform Western blotting PCNA

Get tips on using PCNA Antibody (PC10): sc-56 to perform Western blotting PCNA

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Escherichia coli

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Corynebacterium glutamicum

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Bacillus subtilis