siRNA / RNAi /miRNA transfection Human Cells HeLa

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Fallopian tubes

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human liver tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using pcDNA3.1-MRP4-his6 to perform Protein Expression Eukaryotic cells - HEK293 human MRP4-his6

Get tips on using pET-28b-hBCO1 to perform Protein Expression Prokaryotic cells - E. coli human BCO1

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - human embryonic stem cells

Get tips on using IGEPAL® CA-630 to perform Protein isolation Mammalian cells - Human lung fibroblasts