Protein isolation Mammalian cells

Get tips on using DMEM/F-12 to perform Mammalian cell culture media U251

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media U937

Get tips on using DULBECCO MEM MEDIUM to perform Mammalian cell culture media U87

Get tips on using DULBECCO MEM MEDIUM to perform Mammalian cell culture media T98G

Get tips on using DMEM/F-12 to perform Mammalian cell culture media T47D

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media PNT1A

Get tips on using DMEM/F-12 to perform Mammalian cell culture media A2780

Get tips on using CD CHO Medium to perform Mammalian cell culture media CHO

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.