TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Live-Dead cell staining kit (Enzo) to perform Live / Dead assay mammalian cells - human fibroblast tissue
Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Tissue - Human aortic endothelial cells
Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)
Get tips on using Anti-LC3B antibody (ab48394) to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)
Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - U2OS (human Bone Osteosarcoma Epithelial Cells)
Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human salivary gland stem cells
Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human salivary gland stem cells
Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human intestinal stem cells/organoids
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human neural progenitor cells (NPC)
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