DNA methylation profiling Gene specific profiling Ca Ski

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Get tips on using LightCycler® 480 SYBR Green I Master to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Brilliant II SYBR Green qPCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - CHO-K1

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - COS-7

Get tips on using SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) to perform Microarray Gene expression arrays - Mouse Cyanine-CTP

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.