DNA transfection Mammalian cells Primary cells

Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) to perform Mammalian cell culture media A253

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media A253

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media A172

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media MDCK

Get tips on using DMEM (Dulbecco's Modified Eagle's medium) to perform Mammalian cell culture media DH82

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media DH82

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media Vero

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using pMJS205 to perform Protein Expression Prokaryotic cells - E. coli S. cerevisiae SOX Erv1p