Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - K562 cells
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized 59M
Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR
Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood monocytes
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - rheumatoid arthritis
Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human osteoblasts
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