Protein expression and purification Insect cells Hi5

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using RGS·His Antibody, BSA-free (100ug) to perform Protein tag Detection of His-tagged proteins

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human blood endothelial cell

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized EBL (embryonic lung cell)

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa