Get tips on using Human SCF ELISA Kit (ab100636) to perform ELISA Rat - SC
Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - Human gingival epithelial cells
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using QuantiFluor® RNA System to perform RNA quantification Fuorimetric
Get tips on using PE anti-human CD96 (TACTILE) Antibody to perform Flow cytometry Anti-bodies Human - CD96
Get tips on using FITC Mouse Anti-Human CD51/CD61 to perform Flow cytometry Anti-bodies Human - CD51
Get tips on using PE Mouse Anti-Human CD51/CD61 to perform Flow cytometry Anti-bodies Human - CD51
Get tips on using APC-H7 Mouse Anti-Human CD43 to perform Flow cytometry Anti-bodies Human - CD43
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