rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Mouse - RAW264.7

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using β-Galactosidase Reporter Gene Staining Kit to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using EasySep™ Direct Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma

Get tips on using MACSxpress Whole Blood Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell