The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary arterial smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human hair follicle dermal papilla cells
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons
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