Site Directed Mutagenesis (SDM)

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hSSCs

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell culture media hMSCs

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media hUMSCs

Get tips on using Gibco™ RPMI 1640 Medium to perform Stem cell culture media hHSCs

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell culture media hHSCs

Get tips on using Gibco DMEM/F-12, HEPES to perform Stem cell culture media hMammospheres

Get tips on using MammoCult™ Human Medium Kit to perform Stem cell culture media hMammospheres

Get tips on using Adipogenic Induction Medium to perform Stem cell Differentiation media mPericytes differentiation into adipogenic cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit skeletal muscle-derived stem cells