Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media Human bone mesenchymal stem cell (BMSC)

Get tips on using HyClone Minimal Essential Medium (MEM) variations: Liquid to perform Stem cell culture media Human salivary gland stem cells

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using INTERFERin® to perform siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human osteoblasts - osteoarthritis