siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

Get tips on using 24-Well Cell Invasion Assays, Basement Membrane to perform Cell migration / Invasion cell type - HT-29

Get tips on using Radius™ 24-Well Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y

Get tips on using Vybrant™ MTT Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - FADU

Get tips on using Oris™ Cell Migration Assay - Collagen I Coated to perform Cell migration / Invasion cell type - HaCat

Get tips on using 96-Well Cell Invasion Assay, Basement Membrane to perform Cell migration / Invasion cell type - SK-GT-4

Get tips on using Cell Proliferation Kit I (MTT) to perform

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized A549

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma