Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized PC-3
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized PC-3
Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media PC-3
Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - PC-3
Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - PC-3
Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PC-7
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