Get tips on using iScript™ Reverse Transcription Supermix for RT-qPCR to perform cDNA synthesis Cell lines
Get tips on using Superscript reverse tran-scriptase II (SS RT II) system to perform cDNA synthesis Yeast
Get tips on using BH100bp ladder :: GD 100bp DNA Ladder H3 RTU Ladder to perform DNA Ladder 100 bp
Get tips on using QIAGEN OneStep Ahead RT-PCR Kit (200) to perform PCR Quantitative real-time PCR - Fish species DNA
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.
Get tips on using LC3B antibody to perform Autophagy assay cell type - RT4
Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression HBV RT
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7
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