siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Rat - HO-1

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Concentrate) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MDAH 2774

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin (Concentrate) Clone AE1/AE3 to perform Immunohistochemistry Mouse - Cytokeratin

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - Spleen cells