Protein isolation Mammalian cells

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MCF-7

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media PANC-1

Get tips on using MGMTM-4 Melanocyte Growth Medium-4 BulletKitTM to perform Mammalian cell culture media HEM

Get tips on using EpiLife™ Medium, with 60 µM calcium to perform Mammalian cell culture media HEK

Get tips on using PE anti-mouse CD49b (pan-NK cells) Antibody to perform Flow cytometry Anti-bodies Mouse - CD49b

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using CellGenix® GMP SCGM Stem Cell Growth Medium to perform Mammalian cell culture media NK-92

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Mammalian cell culture media HCAEC

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media RAOEC