siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

Get tips on using pBAD/HisB to perform Protein Expression Prokaryotic cells - E. coli AfFtn

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Get tips on using pIBAINS to perform Protein Expression Prokaryotic cells - E. coli hInsulin

Get tips on using EMBacY#1 to perform Protein Expression Eukaryotic cells - Hi5 mDicer2

Get tips on using EMBacY#6 to perform Protein Expression Eukaryotic cells - Hi5 mCSF1

Get tips on using EMBacY#2 to perform Protein Expression Eukaryotic cells - Hi5 hcAbl