Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.
Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.
Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.
Get tips on using Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893) to perform ChIP Anti-bodies γH2AX
Get tips on using Nutrient agar to perform Bacterial cell culture media Pseudomonas aeruginosa
Get tips on using Nutrient broth to perform Bacterial cell culture media Neisseria meningitides
Get tips on using Yeast Extract to perform Bacterial cell culture media Legionella pneumophilia
Get tips on using CASO Broth to perform Bacterial cell culture media Bordetella bronchiseptica
Get tips on using CHARCOAL AGAR to perform Bacterial cell culture media Bordetella pertussis
Get tips on using Nutrient agar to perform Bacterial cell culture media Staphylococcus aureus
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment