siRNA / miRNA gene silencing Human EM-2

Get tips on using JAM-A CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Deletion F11R

Get tips on using Endothelial Tube Formation Assay (In Vitro Angiogenesis) to perform Angiogenesis assay human - HUVEC

Get tips on using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® to perform RNA sequencing Human - MDA-MB-231

Get tips on using VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MLH1

Get tips on using Androgen Receptor (D6F11) XP® Rabbit mAb #5153 to perform Immunohistochemistry Human - AR

Get tips on using SimpleChIP® Plus Sonication Chromatin IP Kit #56383 to perform ChIP Human - SW480

Get tips on using SimpleChIP® Plus Sonication Chromatin IP Kit #56383 to perform ChIP Human - AGS

Get tips on using EZ‐ChIP™ Assay Kit (Cat#17–371) to perform ChIP Human - HeLa

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.