siRNA / RNAi /miRNA transfection Human Cells Jurkat cells

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Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Human Fallopian tubes

Products Qiagen miRNeasy Mini kit

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human liver tissue

Products Qiagen miRNeasy Mini kit

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Products Qiagen miRNeasy FFPE Kit

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue

Products Qiagen miRNeasy Mini kit

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)

Products Illumina TruSeq Stranded mRNA

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling DU145, PC3 human prostate cancer

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling OVCAR-3 human ovarian cancer

RNA Microarray Human Precision cut lung slices Target preparation kit (RNA Amplification + Hybridization + control)

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Cells - immortalized MA-104

Products Tiangen RNAsimple Total RNA Kit

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