siRNA / miRNA gene silencing Human Min-6

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - SKOV3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HeLa

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunohistochemistry γH2AX - Mouse Human -NA-

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Anti-pan Cytokeratin antibody [C-11] (ab7753) to perform Immunohistochemistry Human - pan Cytokeratin

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Immunohistochemistry Human - β-catenin

Get tips on using MUC5AC Antibody / Mucin-5AC [clone 45M1] (V2198) to perform Immunohistochemistry Human - Muc-5AC

Get tips on using Mucin 2 Antibody (F-2): sc-515032 to perform Immunohistochemistry Human - Muc-2

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - Caco-2

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - THP-1