siRNA / RNAi /miRNA transfection Rat

Get tips on using QuantiNova SYBR Green RT-PCR Kit (2500) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QuantiFast Pathogen RT-PCR +IC Kit (400) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QuantiTect SYBR Green RT-PCR Kit (1000) to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using iScript™ Reverse Transcription Supermix for RT-qPCR to perform cDNA synthesis Cell lines

Get tips on using Superscript reverse tran-scriptase II (SS RT II) system to perform cDNA synthesis Yeast

Get tips on using BH100bp ladder :: GD 100bp DNA Ladder H3 RTU Ladder to perform DNA Ladder 100 bp

Get tips on using QIAGEN OneStep Ahead RT-PCR Kit (200) to perform PCR Quantitative real-time PCR - Fish species DNA

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

Get tips on using RNA Stat-60 RNA Extraction Reagent to perform RNA isolation / purification Cells - immortalized HEK 293

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.