siRNA / miRNA gene silencing Human Human ovarian carcinoma cell (OV2008) Yap Gene

Get tips on using CDX-2 (EPR2764Y) Rabbit Monoclonal Antibody to perform Immunohistochemistry Human - CDX2

Get tips on using ChIP-IT® Express Shearing Kits to perform ChIP Human - SW480

Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2

Get tips on using EpiQuik Acetyl-Histone H4 ChIP Kit to perform ChIP Human - HepG2

Get tips on using truChIP Chromatin Shearing Kit with Formaldehyde to perform ChIP Human - T47D

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - HepaRG human hepatoma

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery endothelial cells