Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Lens Epithelial Cells
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - HK-2 cells
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mel cells
Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - K562 cells
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - K562 cells
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse embryonic fibroblasts
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Corticospinal motor neurons
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity
ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
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