ChIP H3K27me3 Rabbit Donkey

Get tips on using Anti-HDAC1 antibody - ChIP Grade (ab7028) to perform ChIP Anti-bodies HDAC1

Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - SMMC-7721

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - PANC-1

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

Get tips on using Anti Type X Collagen (raised against rat) pAb (Rabbit, Antiserum) to perform Immunohistochemistry Mouse - Col X

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MIA PaCa-2

Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Human - MDA-MB-231

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MDA-MB-231

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.