Immunohistochemistry Wilms Tumor 1 (WT1)

Get tips on using Human CCL2/MCP-1 Quantikine ELISA Kit to perform ELISA Human - MCP1

Get tips on using Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit to perform ELISA Human - ICAM-1/CD54

Get tips on using Anti-TGF beta 1 antibody [TB21] (ab190503) to perform Western blotting TGF-beta1

Get tips on using Anti-AP-1 antibody produced in rabbit to perform Western blotting C-Jun

Get tips on using Mouse IGF-1 PicoKine™ ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using Rat IGF-1 PicoKine™ ELISA Kit to perform ELISA Rat - IGF-I

Get tips on using Human IGF-1 PicoKine™ ELISA Kit to perform ELISA Human - IGF-I

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.