RNA isolation / purification Cells immortalized

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Tissue - Mouse lung tissue

Get tips on using QIAEX II Gel Extraction Kit (150) to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using UltraClean 96 PCR Cleanup Kit (384) to perform DNA gel extraction / PCR product purification PCR clean-up

Get tips on using QIAquick 96 PCR BioRobot Kit (4) to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris EDIII-D1

Get tips on using Zymoclean™ Gel DNA Recovery Kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using ISOLATE II PCR and Gel kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using HiTrap Q FF anion exchange chromatography column to perform Protein expression and purification Bacteria - Bacillus subtilis GCSF